Thermo Fisher Scientific KB-RAS Polyclonal Antibody
Western blot, ELISA, IP 등 다양한 응용에 적합한 Rabbit Polyclonal 항체. Human, Mouse, Rat 반응성. 합성 펩타이드(aa 179-192)로 면역화하여 제작. 1 mg/mL 농도, 액상 형태로 제공. -20°C 보관, 동결-해동 반복 피해야 함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:5,000–1:25,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 179–192 of human KB-RAS. A single amino acid change (S190P) exists in mouse. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Target Information
KappaB ras-1 (kappa-B-ras-1) and kappaB-ras-2 are small proteins similar to Ras-like small GTPases that associate with Ikappa-B, an inhibitor of the transcription factor NF-kappa-B. Ikappa-B exists in two homologous forms, Ikappa-B-alpha and Ikappa-B-beta. Ikappa-B-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive Ikappa-B-alpha–NF-kappa-B complexes can shuttle in and out of the nucleus, Ikappa-B-beta–NF-kappa-B complexes are retained exclusively in the cytoplasm. KappaB-ras proteins preferentially bind to the Ikappa-B-beta form through this unique insert within the ankyrin region, modulating its cellular location and regulating its degradation rate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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