Thermo Fisher Scientific GAB1 Polyclonal Antibody
Rabbit polyclonal antibody against human GAB1 protein. Validated for WB and ICC/IF. Affinity-purified and supplied as liquid form with 0.5 mg/mL concentration. Suitable for research use only. Shows cross-reactivity with monkey, cat, bovine, and sheep.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Rhesus monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptides corresponding to Human GAB1 (aa 524–541, 675–694) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 30% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2633264 |
Product Specific Information
These polyclonal antibodies are of rabbit origin and developed by immunizing animals with specific proteins or peptides. The antibodies are purified by affinity chromatography from rabbit sera and tested for functionality in relevant research applications.
Developed for Research Use Only and are non-hazardous and non-infectious in nature.
This antibody is predicted to react with Monkey, Cat, Bovine, and Sheep.
Target Information
Gab1 is a 115 kDa multiple docking protein essential for cellular growth, transformation, and apoptosis. It can be phosphorylated by multiple receptor tyrosine kinases (RTKs), including insulin receptor (IR), platelet-derived growth factor receptor beta (PDGFRβ), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c-Met), and epidermal growth factor receptor (EGFR), as well as in response to cell-cell adhesion.
Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for PI3K, Grb2, PLCγ1, Nck, and SHP2. Phosphorylation on tyrosines 627 and 659 is critical for SHP2 binding and activation of the ERK/MAPK pathway in response to EGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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