Thermo Fisher Scientific Phospho-SQSTM1 (Ser403) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Flow Cytometry (Flow)

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SQSTM1 (phospho Ser403). The exact sequence is proprietary. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SQSTM1,uniProtId:Q13501-1,ncbiNodeId:9606,antigenRange:403,antigenLength:440,antigenImageFileName:PA5-78267_SQSTM1_Q13501-1_Rabbit.svg,antigenImageFileNamePDP:PA5-78267_SQSTM1_Q13501-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.08 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2736424

Product Specific Information

Positive Control: HepG2 (3µM Thapsigargin treatment for 12hr and 24hr), Huh7 (5 µM MG132), Huh7 (10 µM MG132), HepG2 whole cell lysate/extract (3 µM/mL Thapsigargin treatment for 12hr), HeLa,NG108-15

Predicted Reactivity: Sheep (100%), Rhesus Monkey (100%), Bovine (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.