Thermo Fisher Scientific ABCB1 Monoclonal Antibody (OTI9C10), TrueMAB
ABCB1 단백질을 인식하는 단클론 항체로, Western blot, IHC, Flow Cytometry에 사용 가능. Human, Mouse, Rat 반응성. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도. 약물 내성 연구 및 막단백질 수송 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9C10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 995–1280 of human ABCB1 produced in SF9 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The ABCB1 gene encodes a membrane-associated protein that belongs to the ATP-binding cassette (ABC) transporter superfamily.
ABC transporters move various molecules across cellular membranes and are divided into seven subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).
ABCB1 is part of the MDR/TAP subfamily, involved in multidrug resistance. It acts as an ATP-dependent efflux pump for xenobiotic compounds, reducing intracellular drug accumulation and contributing to anticancer drug resistance.
Additionally, ABCB1 functions as a transporter in the blood-brain barrier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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