Thermo Fisher Scientific Rb Recombinant Rabbit Monoclonal Antibody (SY63-03)
Rb 단백질을 표적으로 하는 재조합 토끼 단일클론 항체로, 높은 특이성과 민감도를 제공합니다. WB, IHC, ICC/IF 등 다양한 응용에 적합하며, HEK293 세포에서 발현된 동물 유래 성분 없는 항체입니다. 안정적인 로트 간 일관성과 연구용 고품질 보증.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY63-03 |
| Immunogen | Recombinant protein within mouse Rb aa 682–921 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809396 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine residues and forms complexes with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E.
Rb protein regulates transcription, and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene are associated with cancers such as breast, colon, prostate, kidney, nasopharynx, and leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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