Thermo Fisher Scientific INCENP Monoclonal Antibody (3D2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 1 publication 1 publication

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3D2

Immunogen

Purified recombinant fragment of human INCENP (amino acids: 369-583) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:INCENP,uniProtId:Q9NQS7-1,ncbiNodeId:9606,antigenRange:369-583,antigenLength:918,antigenImageFileName:MA5-17100_INCENP_Q9NQS7-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17100_INCENP_Q9NQS7-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538571

Product Specific Information

MA5-17100 targets INCENP in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17100 immunogen is purified recombinant fragment of human INCENP (amino acids: 369-583) expressed in E. Coli.

MA5-17100 detects INCENP which has a predicted molecular weight of approximately 105.4kDa.

Target Information

Chromosomal passenger proteins are members of a group of proteins that move from centromeres to the spindle midzone during mitosis. INCENP (Inner Centromere Protein) is a chromosomal passenger protein with an essential role in mitosis and meiosis. It is a complex highly basic multidomain protein that is dynamically translocated from metaphase chromosomes to the spindle midzone during or just prior to anaphase. Some INCENP transfers to the equatorial cortex before formation of the cleavage furrow. Ultimately, it is discarded in the midbody at the completion of cytokinesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.