Thermo Fisher Scientific GATA-3 Recombinant Rat Monoclonal Antibody (TWAJ)
GATA-3 단백질을 인식하는 Rat 유래 재조합 단일클론 항체입니다. Western blot, ICC/IF, Flow Cytometry에 적합하며 높은 특이성과 재현성을 제공합니다. 다양한 형광 표지 형식으로도 제공되며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 0.016–0.25 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgG2b, kappa |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | TWAJ |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Additional Formats
Product Specific Information
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. These antibodies are generated from specific genes encoding the desired antibody, cloned into an expression vector, and expressed in vitro.
Advantages include improved specificity and consistent performance across lots.
It is recommended to titrate the antibody for optimal assay performance.
Target Information
The T-cell antigen receptor (TCR) genes (alpha, beta, gamma, delta) are regulated by distinct enhancers and their enhancer-binding proteins. GATA3 binds to these enhancers, controlling TCR gene expression. GATA3 contains a zinc finger domain and is expressed in T cells but not in B cells or macrophages. It plays a key role in T-lymphocyte lineage differentiation and TCR gene activation. The human GATA3 gene spans 17 kb with 6 exons, encoding two conserved zinc finger domains similar to those of GATA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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