Thermo Fisher Scientific CBF beta Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-317 detects CBF-beta in human samples.
It has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~22 kDa protein representing CBF-beta in HeLa whole cell lysates.

The immunogen is a synthetic peptide corresponding to residues M(1) P R V V P D Q R S K F E N E E F F R K(20) of human CBF beta, which is conserved in mouse.
PA1-317 immunizing peptide (Cat. # PEP-282) is available for use in neutralization and control experiments.

Target Information

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family. It regulates genes involved in hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2).

The beta subunit acts as a non-DNA binding regulatory subunit that enhances DNA binding of the alpha subunit. The complex binds to core sites of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, and GM-CSF promoters.

Alternative splicing generates two mRNA variants encoding distinct carboxyl termini. A pericentric inversion of chromosome 16 [inv(16)(p13q22)] can produce a chimeric transcript involving core-binding factor beta and smooth muscle myosin heavy chain 11, associated with acute myeloid leukemia (M4Eo subtype).

Two transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.