Thermo Fisher Scientific DNMT3L Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (IHC)

1:500

-

ELISA (ELISA)

1:1,000-1:3,000

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

synthetic peptide C-GLLQRRRKWRSQL conjugated to KLH, corresponding to amino acids 152-164 of Human DNMT 3L.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2094277

Product Specific Information

PA1-31714 detects Dnmt3L from human samples.

Target Information

Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development. Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus. These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B. DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.