Thermo Fisher Scientific APC10 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,500

-

Immunohistochemistry (IHC)

1:250-1:1,000

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

synthetic peptide GADPKQLERTG, corresponding to amino acids 10-20 of Human Apc10.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2226991

Product Specific Information

PA1-31765 detects APC10 from human samples.

Target Information

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC10 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. It has been suggested that APC10 plays a role to regulate the binding of specific substrates to the APC/C complex, similar to that of coactivators.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.