Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Super Bright 645, eBioscience
마우스 CD4 분자를 인식하는 RM4-5 클론 단일클론 항체로, Super Bright 645 형광체가 결합된 제품입니다. Flow Cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되어 645 nm에서 방출됩니다. 높은 신호 선명도와 안정성을 제공하며, 연구용으로 적합합니다.
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Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Super Bright 645, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 8 publications |
| Immunohistochemistry (PFA fixed) | - | View 1 publication |
| Immunohistochemistry (Frozen) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 70 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RM4-5 |
| Conjugate | Super Bright™ 645 |
| Excitation / Emission Max | 414 / 645 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2662400 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV (395~805)
- Brilliant Violet (421~786)
- eFluor (450, 506, 570, 615)
- FITC
- NovaFluor Blue / Yellow series
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright (436, 600, 702, 780)
- Custom conjugation available
Product Specific Information
The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, a subset of mature T cells, and dendritic cells. CD4 binds to MHC class II molecules and plays a critical role in T cell development and activation. RM4-5 binding is blocked by GK1.5.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse thymocytes and splenocytes at ≤0.25 µg/test
- Excitation / Emission: 405 nm / 645 nm (Violet Laser)
- Recommended Filter: 660/20 bandpass
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.
Light Sensitivity
Protect vial and stained samples from light due to photo-induced oxidation.
Fixation
Samples can be stored up to 3 days at 4°C in the dark using:
- IC Fixation Buffer (Product # 00-8222)
- 1-step Fix/Lyse Solution (Product # 00-5333)
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD4 antigen is a co-receptor for MHC class II molecules and HIV. It is primarily expressed in T helper cells, as well as monocytes, macrophages, and dendritic cells. Tissue-level expression includes thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut. CD4 facilitates early T-cell activation via association with the T-cell receptor complex and Lck kinase. Multiple alternatively spliced transcripts exist (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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