Thermo Fisher Scientific Phospho-NLRC4 (Ser533) Polyclonal Antibody
Phospho-NLRC4 (Ser533) 인산화 단백질을 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot에 적합하며 Human, Mouse, Rat 시료에서 반응. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-NLRC4 (Ser-533) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding serine 533 in mouse NLRC4. This sequence has high homology to human and rat NLRC4, and is not homologous to sequences in other NLR family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942956 |
Product Specific Information
The antibody was cross-adsorbed to unphosphorylated NLRC4 (Ser-533) peptide before affinity purification using phospho-NLRC4 (Ser-533) peptide.
This antibody detects a 110 kDa* protein on SDS-PAGE immunoblots of PMA-differentiated THP1 human macrophages.
Additionally, the antibody detects a mouse recombinant phospho-NLRC4 (Ser-533) peptide but does not detect this peptide after lambda phosphatase treatment.
Target Information
NLRC4 encodes a member of the caspase recruitment domain-containing NLR family.
Family members play essential roles in the innate immune response to a wide range of pathogenic organisms, tissue damage, and other cellular stresses.
Mutations in this gene result in autoinflammation with infantile enterocolitis.
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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