Thermo Fisher Scientific VGAT Monoclonal Antibody (D9), FITC
VGAT 단백질을 인식하는 마우스 단일클론 항체로 FITC 형광 표지. Western blot, IHC, ELISA에 사용 가능. 498/517 nm의 형광 특성. 4°C 암소 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | D9 |
| Immunogen | Synthetic peptide from the N-terminal region of Mouse VGAT protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:500 dilution of MA5-45925 was sufficient for detection of VGAT in 15 µg of Rat Brain Membrane by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects 57 kDa.
Target Information
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Acid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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