Thermo Fisher Scientific VGAT Monoclonal Antibody (D9), APC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

D9

Immunogen

Synthetic peptide from the N-terminal region of Mouse VGAT protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:VGAT,uniProtId:O35633-1,ncbiNodeId:10090,antigenRange:1,antigenLength:525,antigenImageFileName:MA5-45924_VGAT_O35633-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45924_VGAT_O35633-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

A 1:500 dilution of MA5-45924 was sufficient for detection of VGAT in 15 ug of Rat Brain Membrane by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects 57 kDa.

Target Information

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.