Thermo Fisher Scientific Cytochrome C Recombinant Mouse Monoclonal Antibody (rCYCS, 1010)
Cytochrome C 단백질을 인식하는 Thermo Fisher Scientific의 재조합 마우스 단클론 항체. Western blot, IHC, Flow cytometry 등 다양한 응용 가능. 높은 특이성과 안정적인 결과 제공. 연구용으로만 사용.
- 카탈로그번호
- 54205-MSM7-P1ABX
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Amphibian, Avian, Dog, Fruit fly, Horse, Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rCYCS, 1010 |
| Immunogen | Synthetic peptides corresponding to amino acid 1–80, 81–104 and 66–104 of pigeon cytochrome c |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C or −80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C followed by cooling at room temperature for 20 minutes.
Target Information
Cytochrome C is an electron transporting protein located within the intermembrane space of mitochondria, playing a critical role in oxidative phosphorylation and ATP production. Release of cytochrome C into the cytosol is triggered by apoptotic stimuli, where it binds apoptotic protease activating factor, activating procaspase 9. Cytosolic cytochrome C also activates caspase 3, a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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