Thermo Fisher Scientific Phospho-p27 Kip1 (Thr187) Polyclonal Antibody
p27 Kip1 단백질의 Thr187 위치 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC 등 다양한 응용에 적합. 고순도 액상 형태로 제공되며 PBS/glycerol buffer에 안정적으로 보관 가능. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CDKN1B (Accession P46527), corresponding to amino acid residues around phosphorylated Thr187 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816384 |
Product Specific Information
This antibody detects endogenous levels of p27 Kip1 only when phosphorylated at Threonine 187.
Target Information
p27 Kip1 functions as a negative regulator of G1 phase expression and may mediate TGFβ-induced G1 arrest. It regulates cell cycle progression by inhibiting cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 also interacts with cyclin D-CDK4 complexes, influencing their assembly and activation depending on its phosphorylation state. The protein (~27 kDa) undergoes CDK-dependent phosphorylation and ubiquitination by SCF complexes, which triggers degradation necessary for the transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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