Thermo Fisher Scientific Phospho-RHOA (Thr100) Polyclonal Antibody
RhoA 단백질의 Thr100 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(Paraffin) 분석에 적합. Human, Mouse, Rat 시료 반응성. PBS/glycerol buffer에 보관되며, 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human RHOA (Accession P61586), corresponding to amino acid residues around phosphorylated Thr100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817026 |
Product Specific Information
This antibody detects endogenous levels of RhoA only when phosphorylated at Thr100.
Target Information
RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal required for myosin contractile ring formation during cytokinesis and plays an essential role in cleavage furrow formation. RhoA is necessary for apical junction formation in keratinocyte cell-cell adhesion and acts as a target for the yopT cysteine peptidase from Yersinia pestis and Yersinia pseudotuberculosis. It stimulates PKN2 kinase activity and may activate PLCE1. RhoA is activated by ARHGEF2, promoting GDP-GTP exchange, and is essential for SPATA13-mediated regulation of cell migration and adhesion dynamics. The MEMO1-RHOA-DIAPH1 signaling pathway is crucial for ERBB2-dependent microtubule stabilization at the cell cortex, controlling APC and CLASP2 localization via GSK3B regulation. Membrane-bound APC enables MACF1 localization, required for microtubule capture and stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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