Thermo Fisher Scientific A33 Monoclonal Antibody (402104)
A33 단백질을 표적으로 하는 Rat IgG2a 단클론 항체로, Western blot, IHC, Flow Cytometry에 적합. 인간 시료 반응성, 비보존제 PBS 기반 완충액, 동결건조 형태로 제공. 재구성 후 0.5 mg/mL 농도로 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | 8–25 µg/mL | - |
| Flow Cytometry (Flow) | 2.5 µg per million cells | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 402104 |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human A33 (Ile22–Val235) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2605828 |
Product Specific Information
- In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse A33 is observed.
- Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
The glycoprotein encoded by this gene is a cell surface antigen expressed in over 95% of human colon cancers.
It encodes a 319-amino acid polypeptide with a secretory signal sequence and three potential glycosylation sites.
The mature protein includes a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail.
The extracellular region contains two domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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