Thermo Fisher Scientific Calbindin D28K Monoclonal Antibody (401025)
Calbindin D28K 단백질을 인식하는 Thermo Fisher Scientific의 마우스 모노클로날 항체로, Western blot 및 Immunohistochemistry에 적합. 인간 시료에 반응하며, 단백질 A/G로 정제된 동결건조 형태. PBS와 5% trehalose 버퍼에 보관, -20°C에서 안정적.
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Thermo Fisher Scientific Calbindin D28K Monoclonal Antibody (401025)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.25–10 µg/mL | - |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 8–25 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 8–25 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 401025 |
| Immunogen | E. coli-derived recombinant human Calbindin D Met1–Asn261 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609695 |
Product Specific Information
- In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human S100P.
- Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin) is a highly conserved 28 kDa calcium-binding protein with broad tissue distribution.
It belongs to a family of low molecular weight calcium-binding proteins (CaBPs), including calmodulin, S-100, parvalbumin, and troponin C.
These proteins share homologous primary structures with conserved EF-hand motifs for Ca²⁺ binding.
Calbindin-D-28K is predominantly found in subpopulations of central and peripheral nervous system neurons, as well as epithelial cells involved in Ca²⁺ transport (e.g., distal tubular cells and cortical collecting tubules of the kidney, and enteric neuroendocrine cells).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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