Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Super Bright 600, eBioscience
마우스 CD19를 인식하는 eBio1D3 (1D3) 단클론 항체로, Super Bright 600 형광체로 결합됨. Flow cytometry에 최적화되어 있으며, B 세포 발현 분석에 적합. 405 nm 레이저로 여기되어 600 nm에서 방출. 4°C 암소 보관, 동결 금지.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 53 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host/Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio1D3 (1D3) |
| Conjugate | Super Bright™ 600 |
| Excitation/Emission Max | 414/601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637308 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV/Violet series
- eFluor 450, 506, 660
- FITC, PE, PE-Cyanine series
- PerCP-Cyanine5.5
- Super Bright 436, 645, 702, 780
- NovaFluor Blue/Red/Violet/Yellow series
- Request custom conjugation
Product Specific Information
The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except plasma cells. Follicular dendritic cells also express CD19. CD19 forms a complex with CD21, CD81, and MHC class II, associating with the BCR to regulate signaling, calcium flux, and proliferation of B cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes, ≤0.5 µg/test (100 µL final volume). Cell count: 10⁵–10⁸ cells/test. Optimize titration for best performance.
Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended detection filter: 610/20 bandpass. Use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions when multiple Super Bright dyes are used.
Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored up to 3 days at 2–8°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) with minimal impact on brightness.
Excitation/Emission: 405 nm / 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and on certain myeloid leukemia cells. CD19 serves as a key regulator of B cell activation and proliferation, functioning as a signal-amplifying coreceptor for the B cell receptor (BCR). Mutations in CD19 can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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