Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), Super Bright 600, eBioscience
인간 CD19 항원을 인식하는 SJ25C1 단클론 항체로, B세포 분석용 Super Bright 600 형광 접합체입니다. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되어 600 nm에서 방출합니다. 빛에 민감하며 4°C 암소 보관 권장.
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Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), Super Bright 600, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 11 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 600 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SJ25C1 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637472 |
Available Formats
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 615, 737, 805
- Brilliant Violet 605, 650
- eFluor 450, 506
- NovaFluor Blue 610-70S, 660-120S, 690
- NovaFluor Red 685, 725, 755
- NovaFluor Yellow 590, 610, 690, 755
- PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except plasma cells. CD19 forms a complex with CD21, CD81, Leu13, and MHC class II, associating with the BCR to mediate signaling and proliferation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended 5 µL (0.5 µg) per test (100 µL final volume).
- Excitation/Emission: 405 nm / 600 nm (Violet Laser)
- Filter Recommendation: 610/20 bandpass filter
- Light Sensitivity: Protect from light to avoid photo-induced oxidation
- Fixation: Compatible with IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 2–8°C in dark
- Buffer Recommendation: Use Super Bright Complete Staining Buffer (Product #SB-4401) when multiple Super Bright dyes are used
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD19 is an immunoglobulin superfamily member with two Ig-like domains, expressed on all peripheral B cells and some myeloid leukemia cells. It regulates B cell proliferation and differentiation, acting as a co-receptor with the BCR to amplify antigen signaling. CD19 mutations cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures.
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