Thermo Fisher Scientific Phospho-beta Catenin/gamma Catenin (Tyr654, Tyr644) Polyclonal Antibody
Phospho-beta Catenin/gamma Catenin (Tyr654, Tyr644)에 특이적인 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB 및 ICC/IF에 사용 가능하며, 인간, 마우스, 랫트 반응성. 고순도 항원 친화 크로마토그래피 정제, 안정적 액상 형태로 -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-beta-Catenin (Tyr-654) synthetic peptide corresponds to amino acid residues around tyrosine 654 of human beta-Catenin. This sequence is highly conserved in rat, mouse, chicken, and frog beta-Catenins, and homologous to gamma-Catenin (Tyr-644). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942895 |
Product Specific Information
This antibody was cross-adsorbed to phospho-beta-Catenin (Tyr-86) peptide before affinity purification using phospho-beta-Catenin (Tyr-654) peptide (without carrier).
It detects 84 and 88 kDa proteins corresponding to gamma-Catenin and beta-Catenin, respectively, on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but does not detect these proteins in control cells.
Target Information
Beta-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. Cadherins are transmembrane adhesion molecules found with catenins at adherens junctions.
Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in loss of cell adhesion. Tyrosine phosphorylation of beta-Catenin regulates its interaction with adherens junction components.
Fer and Fyn kinases phosphorylate tyrosine 142 in vitro, disrupting alpha- and beta-Catenin interactions when overexpressed. Phosphorylation at tyrosine 142 may act as a switch between transcriptional and adhesive roles of beta-Catenin.
Src family kinases can also phosphorylate tyrosine 86 and 654 in beta-Catenin. Tyr-654 phosphorylation regulates beta-Catenin binding to E-Cadherin, influencing cell adhesion through site-specific tyrosine phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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