Thermo Fisher Scientific BCKDHA Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Product Specifications

Product Specific Information

• Positive test controls: Mouse liver, Mouse kidney, Mouse heart, Rat kidney, Rat liver
• Target location: Mitochondrion matrix

Immunogen sequence:
SEQYRGDGIA ARGPGYGIMS IRVDGNDVFA VYNATKEARR RAVAENQPFL IEAMTYRIGH HSTSDDSSAY RSVDEVNYWD KQDHPISRLR HYLLSQGWWD EEQEKAWRKQ SRRKVMEAFE QAERKPKPNP NLLFSDVYQE MPAQLRKQQE SLARHLQTYG EHYPLDHFDK

Target Information

The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes oxidative decarboxylation of branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of branched-chain amino acids.

The BCKD complex consists of:

• A heterotetrameric (α2-β2) branched-chain alpha-keto acid decarboxylase (E1)
• A homo-24-meric dihydrolipoyl transacylase (E2)
• A homodimeric dihydrolipoamide dehydrogenase (E3)

E1 is a thiamine pyrophosphate (TPP)-dependent enzyme, and the reaction is irreversible, constituting the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains two regulatory enzymes: a kinase and a phosphorylase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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