Thermo Fisher Scientific BCKDHA Polyclonal Antibody
Rabbit polyclonal antibody targeting human BCKDHA, validated for Western blot. Recognizes mitochondrial matrix protein involved in branched-chain amino acid metabolism. Supplied as liquid, unconjugated form, 0.2 mg/mL concentration, stored at -20°C.
- 카탈로그번호
- PA5120486
- 판매단위
- pk
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 276–445 of human BCKDHA (NP_000700.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914058 |
Product Specific Information
- Positive test controls: Mouse liver, Mouse kidney, Mouse heart, Rat kidney, Rat liver
- Target location: Mitochondrion matrix
Immunogen sequence:
SEQYRGDGIA ARGPGYGIMS IRVDGNDVFA VYNATKEARR RAVAENQPFL IEAMTYRIGH HSTSDDSSAY RSVDEVNYWD KQDHPISRLR HYLLSQGWWD EEQEKAWRKQ SRRKVMEAFE QAERKPKPNP NLLFSDVYQE MPAQLRKQQE SLARHLQTYG EHYPLDHFDK
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes oxidative decarboxylation of branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of branched-chain amino acids.
The BCKD complex consists of:
- A heterotetrameric (α2-β2) branched-chain alpha-keto acid decarboxylase (E1)
- A homo-24-meric dihydrolipoyl transacylase (E2)
- A homodimeric dihydrolipoamide dehydrogenase (E3)
E1 is a thiamine pyrophosphate (TPP)-dependent enzyme, and the reaction is irreversible, constituting the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains two regulatory enzymes: a kinase and a phosphorylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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