Thermo Fisher Scientific Caspase 3 Polyclonal Antibody
Caspase-3 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, ELISA, IP에 사용 가능. Human, Mouse, Rat 반응성. 액상 형태로 2.83 mg/mL 농도, PBS/glycerol 버퍼에 보관. 세포사멸 연구 및 단백질 발현 분석에 적합.
- 카탈로그번호
- PA5120488
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Caspase 3 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 29–175 of human Caspase-3 (NP_0043372) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.83 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914060 |
Product Specific Information
- Positive test controls: HeLa, 293T, NIH/3T3, Jurkat
- Subcellular location: Cytoplasm
- Immunogen sequence:
SGISLDNSYK MDYPEMGLCI IINNKNFHKS TGMTSRSGTD VDAANLRETF RNLKYEVRNK NDLTREEIVE LMRDVSKEDH SKRSSFVCVL LSHGEEGIIF GTNGPVDLKK ITNFFRGDRC RSLTGKPKLF IIQACRGTEL DCGIETD
Target Information
This gene encodes a protein that belongs to the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues, producing large and small subunits that dimerize to form the active enzyme.
Caspase-3 cleaves and activates caspases 6, 7, and 9, and is itself processed by caspases 8, 9, and 10. It is a predominant caspase involved in cleavage of amyloid-beta precursor protein, associated with neuronal death in Alzheimer’s disease.
Alternative splicing results in two transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.