Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), Super Bright 702, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 661, 805
• Brilliant Violet 421, 650, 711
• eFluor 450, 506, 570, 660
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor belonging to the EGF-TM7 protein family. The F4/80 antigen is a well-established marker for mature macrophages, though it may also be expressed by peritoneal eosinophils, Langerhans cells, and certain dendritic cell subsets. Expression begins early in myeloid development and can be upregulated by M-CSF stimulation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis of mouse resident peritoneal exudate cells. Recommended ≤1.0 µg/test (100 µL final volume).
• Excitation: 405 nm
• Emission: 702 nm
• Laser: Violet Laser

Super Bright 702 is a tandem dye excited with the violet laser (405 nm) and emits at 702 nm. Use a 710/50 bandpass filter. When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal signal loss.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein, a member of the EGF-TM7 family, sharing 68% amino acid identity with human EMR1. Expression varies during macrophage maturation and activation. It is expressed on macrophages in tissues such as liver (Kupffer cells), skin (Langerhans), brain (microglia), gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma, and spleen red pulp. It may also appear on certain dendritic cells but is absent from macrophages in T cell zones of lymphoid organs. F4/80 may play a role in the generation of efferent CD8⁺ regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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