Thermo Fisher Scientific c-Myc Chimeric Recombinant Mouse Monoclonal Antibody (9E10)

Applications

• Western Blot (WB): Assay-dependent
• Immunohistochemistry (Paraffin) (IHC (P)): Assay-dependent
• Immunohistochemistry (Frozen) (IHC (F)): Assay-dependent
• Immunocytochemistry (ICC/IF): Assay-dependent
• Immunoprecipitation (IP): Assay-dependent

Product Specifications

Product Specific Information

Epitope sequence: EQKLISEEDL, located in aa 410–419 of human c-myc protein.

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a critical role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.
Phosphorylation of c-Myc at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is associated with cell proliferation and cycle regulation.
c-Myc is essential for vasculogenesis and angiogenesis during tumor development. Deregulated expression induces apoptosis in different cell types.
Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to amino- or carboxy-termini of target proteins.
Amplification of the c-Myc gene occurs in several human tumors, including lung, breast, and colon carcinomas, while N-Myc amplification is found in neuroblastomas.
Chromosomal translocations involving c-Myc are described in Burkitt’s lymphoma and other B-cell lymphoproliferative conditions.
Aberrant expression of c-Myc is observed in tumors of diverse origins such as colorectal, gastric, hepatic, ovarian, pulmonary, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.