Thermo Fisher Scientific Tyrosinase Monoclonal Antibody (TYR, 3829)
Tyrosinase 단백질을 인식하는 Mouse 단일클론 항체로, IHC, ICC/IF, Flow Cytometry, Peptide Array 등 다양한 응용에 적합합니다. Human 반응성, Unconjugated 형태이며, 고순도 Protein A/G 정제. 멜라닌 생합성 연구 및 피부색 관련 유전 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TYR, 3829 |
| Immunogen | Recombinant fragment (around aa332–474) of human Tyrosinase protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C or −80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Tyrosinase is an enzyme that catalyzes the first two steps and at least one subsequent step in the conversion of tyrosine to melanin. It exhibits both tyrosine hydroxylase and dopa oxidase activities and requires copper for function. Mutations in this gene cause oculocutaneous albinism, while polymorphisms contribute to skin pigmentation variation. The human genome also contains a pseudogene similar to the 3′ half of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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