Thermo Fisher Scientific GFP Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse, or Rat serum proteins.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.

As a fusion tag, GFP can be used to localize proteins, study their movement, or research the dynamics of subcellular compartments. GFP technology has provided new insights into the physiological activities of living cells. GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (maximally at 395 nm) and emits green light (peak emission 508 nm) without additional proteins, substrates, or cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications such as monitoring gene expression, protein localization, assessing protein–protein interactions (yeast two-hybrid system), and measuring distances between proteins in FRET experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.