Thermo Fisher Scientific GFP Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot contents and freeze at -20°C or below for extended storage.
• Avoid repeated freeze/thaw cycles.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Assay Information:
Immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified or partially purified Green Fluorescent Protein (Aequorea victoria).
No reaction was observed against Human, Mouse, or Rat serum proteins.

Target Information

The jellyfish Aequorea victoria contains Green Fluorescent Protein (GFP Tag) that emits light in a bioluminescence reaction. GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular systems.

As a fusion tag, GFP enables localization studies, movement tracking, and research on subcellular dynamics. GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically. It absorbs blue light (max 395 nm) and emits green light (peak 508 nm) without requiring additional cofactors.

GFP fluorescence is stable, species-independent, and suitable for diverse applications such as monitoring gene expression, protein localization, protein–protein interaction (yeast two-hybrid), and fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.