Thermo Fisher Scientific Bestrophin 1 Monoclonal Antibody (E6-6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-Dependent

-

in situ PLA (PLA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Pig

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

E6-6

Immunogen

Synthetic peptide conjugated to KLH corresponding to the C-terminus of human Bestrophin 1 (KDHMDPYWALENRDEAHS) [Uniprot: O76090] (aa 568-585). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Bestrophin 1,uniProtId:O76090-1,ncbiNodeId:9606,antigenRange:568-585,antigenLength:585,antigenImageFileName:MA1-16739_Bestrophin_1_O76090-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16739_Bestrophin_1_O76090-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568460

Product Specific Information

This antibody does not react with rat samples.

Suggested positive control: Pig RPE (retinal Pigment Epithelium) whole cell extract.

Target Information

The retinal pigment epithelium (RPE) and choroid represent a differentiated system of the eye that sustains normal retinal health and function. Best vitelliform macular dystrophy, known as Best disease, is an early-onset autosomal dominant condition in which accumulation of lipofuscin-like material within and beneath the RPE leads to progressive loss of central vision. The lipofuscin-like material in the macular area appears as a yellow mass like the yolk of an egg that later becomes darker and irregular in color, a process known as scrambling the egg. Best disease is frequently a reflection of mutations in the Bestrophin gene, which encodes a protein containing four putative transmembrane domains and localizes to the basolateral plasma membrane of RPE cells. Human Bestrophin forms oligomeric chloride channels that are sensitive to intracellular calcium. Missense mutations at the Bestrophin locus reduces or abolishes Bestrophin protein mediated membrane current. The human Bestrophin gene encodes a 585 amino acid protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.