Thermo Fisher Scientific Calnexin Polyclonal Antibody
Calnexin 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, Flow cytometry 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 고순도 친화성 크로마토그래피 정제, 액상 형태로 안정적 보관 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within C-terminal residues of rat Calnexin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2913239 |
Product Specific Information
- Positive Control: Daudi, mouse lung tissue, human colon tissue, human breast cancer tissue, human kidney tissue, mouse testis tissue, SKOV-3
- Subcellular Location: Endoplasmic reticulum
Target Information
Calnexin, also referred to as IP90, p88, and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). It acts as a molecular chaperone aiding in proper folding and assembly of polypeptides. Calnexin associates with MHC class I heavy chains and partial complexes of T cell receptor and B cell membrane immunoglobulin. It retains incompletely folded proteins in the ER. Soluble ER proteins often contain the KDEL sequence for retention, while integral membrane proteins like calnexin rely on positively charged cytosolic residues for ER localization. Calnexin structure includes a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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