Thermo Fisher Scientific RIPK1 Monoclonal Antibody (OTI1A7), TrueMAB

Applications

• Western Blot (WB): 1:500 dilution tested

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

RIPK1 is a serine-threonine kinase that mediates inflammatory and programmed necrosis signaling following activation of death receptors, pathogen recognition receptors (PRRs), or DNA damage.
Upon TNFR1 activation by TNF-alpha cytokines, TRADD and TRAF2 are recruited, leading to phosphorylation of DAB2IP at Ser-728 and activation of the MAP3K5-JNK apoptotic cascade.
Ubiquitination by TRAF2 via Lys-63-linked chains enhances downstream signaling through MAPK and NF-κB pathways, which activate genes encoding inflammatory molecules.
Polyubiquitinated RIPK1 binds to IKBKG/NEMO, a key step for NF-κB activation. Interaction with RHIM-containing adapters can trigger gene activation and cell death.
RIPK1-RIPK3 association forms a necrosis-inducing complex.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.