Thermo Fisher Scientific MPR 300 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: MPRI.

Target Information

Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are transported within the cell by approximately 300 kDa cation-independent mannose 6-phosphate receptors (CI-MPR).
The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans-Golgi network to late endosomes or early lysosomes.
The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes.
The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady-state distribution of MPRs is predominantly within late endosomes, fewer in the trans-Golgi network, and approximately 10% at the cell surface.
In addition to its man6P binding activity, the MPR contains a separate binding site for type II insulin-like growth factor (IGF-II) and can bind both man6P and IGF-II simultaneously.
An approximately 240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.