Thermo Fisher Scientific Protein APC Monoclonal Antibody (FE9)
Human, Mouse, Rat에 반응하는 단클론 항체로 Western blot에 적합. APC 단백질의 N-말단 35 아미노산을 인식하며, Protein G로 정제된 액상 형태. 종양 억제 단백질 연구 및 Wnt 신호전달 경로 분석에 활용 가능.
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Applications
- Western Blot (WB)
Tested Dilution: 1–1.5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FE9 |
| Immunogen | Synthetic peptide corresponding to the N-terminal 35 amino acid residues of APC |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 0.05M sodium phosphate, pH 7.5, with 0.2% gelatin |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_1954862 |
Product Specific Information
- Reacts with all full-length and truncated APC proteins.
- Successfully used in Western blot applications; expected band size ≈ 312 kDa.
- Suggested positive controls: HCT116 cells (p300) and SW480 cells (truncated APC, p147).
Target Information
This gene encodes a tumor suppressor protein that antagonizes the Wnt signaling pathway. It is also involved in cell migration, adhesion, transcriptional activation, and apoptosis.
Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that can progress to malignancy.
Disease-associated mutations are often clustered in the mutation cluster region (MCR), resulting in truncated protein products.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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