Thermo Fisher Scientific K-Ras Monoclonal Antibody (F132-62)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

4-6 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

4-6 µg/mL

-

Immunocytochemistry (ICC/IF)

1.5-2.5 µg/mL

-

Immunoprecipitation (IP)

3-5 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat, Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

F132-62

Immunogen

Recombinant full length human protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:K-Ras,uniProtId:P01116-1,ncbiNodeId:9606,antigenRange:1-189,antigenLength:189,antigenImageFileName:MA1-91046_K-Ras_P01116-1_House_mouse.svg,antigenImageFileNamePDP:MA1-91046_K-Ras_P01116-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

0.05M sodium phosphate with 0.2% gelatin

Contains

0.1% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_1959171

Product Specific Information

Recommended positive controls: Sw480 or Y1 cells or normal skin tissue..

Target Information

K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.