Thermo Fisher Scientific SRC Recombinant Rabbit Monoclonal Antibody (7G6M9)
SRC 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, ICC, ELISA, IP 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. HEK293 세포 발현, 친화 크로마토그래피 정제. PBS 기반 보존 용액, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 400–600 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7G6M9 |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human Src (P12931) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.62 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911826 |
Product Specific Information
- Positive test controls: HeLa, Jurkat, Mouse brain, Mouse lung, Rat brain
- Subcellular localization: Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, Cytoskeleton
Immunogen sequence:
MGSNKSKPKD ASQRRRSLEP AENVHGAGGG AFPASQTPSK PASADGHRGP SAAFAPAAAE PKLFGGFNSS DTVTSPQRAG PLAGGVTTFV ALYDYESRTE
Target Information
SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. It regulates growth and differentiation of eukaryotic cells by associating with cytoplasmic regions of various cell surface receptors to facilitate signal transduction. SRC also plays roles in cytoskeletal organization, cell cycle regulation, and apoptosis.
In humans, the SRC gene is located on chromosome 20. Activity is regulated by phosphorylation of Tyr416 and Tyr527, which have opposing effects on enzyme activity. Mutations in SRC may contribute to malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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