Thermo Fisher Scientific Phospho-HSF1 (Ser303) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Detects endogenous HSF1 protein at approximately 82 kDa.
• Purity >95% by SDS-PAGE.

Target Information

All organisms respond to elevated temperatures and various environmental stresses by the rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator heat shock factor (HSF), which binds to heat shock response elements (HSEs).
These HSEs are arranged as three repeats of a 5-nucleotide {nGAAn} module and are highly conserved among species.
Human cells contain two HSFs, HSF1 and HSF2, sharing 38% sequence identity. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA-binding activity. Upon stress, HSF1 is activated to a nuclear-localized trimeric form that binds DNA. Phosphorylation of HSF1 is required for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.