Thermo Fisher Scientific Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), Super Bright 436, eBioscience
마우스 Ly-6G/Ly-6C 항원을 인식하는 RB6-8C5 클론 단클론 항체로, Super Bright 436 형광체로 결합되어 있습니다. 골수 및 말초 혈액 내 과립구 및 단핵구 분석에 적합하며, 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), Super Bright 436, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 7 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 23 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RB6-8C5 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2735068 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 805
- Brilliant Violet 421 / 650 / 711 / 786
- eFluor 450 / 506 / 660
- FITC
- Functional Grade
- NovaFluor Red 700 / Yellow 610
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, also known as the myeloid differentiation antigen Gr-1 (21–25 kDa). Gr-1 is a GPI-linked protein expressed in a developmentally regulated manner by the myeloid lineage in bone marrow.
Monocytes express Gr-1 transiently during development, while granulocytes and peripheral neutrophils show consistent expression, making Gr-1 a reliable marker for these populations.
Testing by eBioscience indicates that RB6-8C5 also stains cells expressing high levels of Ly6c. For Ly-6G-specific targets, use 1A8-Ly6G (Product # 9668).
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse bone marrow cells at ≤0.5 µg/test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test. Titrate for optimal performance.
Super Bright 436 is excited at 405 nm (violet laser) and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are licensed from Becton, Dickinson and Company.
Target Information
Granulocytes are white blood cells containing cytoplasmic granules. Also known as polymorphonuclear leukocytes (PMN or PML), they exhibit lobed nuclei, typically with three segments. The term PMN often refers specifically to neutrophils, the most abundant granulocytes. These cells are released from bone marrow under regulation by complement proteins.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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