Thermo Fisher Scientific Ly-6C Monoclonal Antibody (HK1.4), Super Bright 436, eBioscience

Thermo Fisher Scientific Ly-6C Monoclonal Antibody (HK1.4), Super Bright 436, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test
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Product Specifications

Available Formats:

• Alexa Fluor 488
• APC
• APC-eFluor 780
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Custom conjugation available

Product Specific Information

The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family.
Ly-6C is expressed on monocytes/macrophages, endothelial cells, granulocytes, and a subset of lymphocytes.
Expression varies among mouse strains:

• Ly6c.1 (C57Bl/6, SJL): stains both CD4 and CD8 cells
• Ly6c.2 (BALB/c, 3H/He): stains CD8 but not CD4 cells

In vitro, HK1.4 antibody can increase proliferation and stimulate cytokine release.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL volume, 10⁵–10⁸ cells/test).
Titrate antibody for optimal performance.

Fluorochrome Information:
Super Bright 436 is excited with the violet laser (405 nm) and emits at 436 nm.
Recommended filter: 450/50 bandpass or equivalent.
Ensure your instrument is compatible with this fluorochrome.

Buffer Recommendation:
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize nonspecific interactions.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product # 00-8222)
• 1-step Fix/Lyse Solution (Product # 00-5333)
  Storage: up to 3 days at 4°C in the dark with minimal signal loss.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Granulocytes are white blood cells containing cytoplasmic granules, also known as polymorphonuclear leukocytes (PMN/PML) due to their lobed nuclei.
They include neutrophils, the most abundant granulocytes, and are released from bone marrow under the regulation of complement proteins.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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