Thermo Fisher Scientific Ly-6C Monoclonal Antibody (HK1.4), Super Bright 436, eBioscience
마우스 Ly-6C 단백질을 인식하는 HK1.4 단클론 항체로, Super Bright 436 형광체로 결합되어 있습니다. 유세포 분석(Flow Cytometry)에 적합하며, 405 nm 레이저로 여기되어 436 nm에서 방출됩니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific Ly-6C Monoclonal Antibody (HK1.4), Super Bright 436, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 1.0 µg/test
- View 43 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Mouse |
| Host / Isotype | Rat / IgG2c, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HK1.4 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2735066 |
Available Formats:
- Alexa Fluor 488
- APC
- APC-eFluor 780
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-Cyanine5.5
- Custom conjugation available
Product Specific Information
The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family.
Ly-6C is expressed on monocytes/macrophages, endothelial cells, granulocytes, and a subset of lymphocytes.
Expression varies among mouse strains:
- Ly6c.1 (C57Bl/6, SJL): stains both CD4 and CD8 cells
- Ly6c.2 (BALB/c, 3H/He): stains CD8 but not CD4 cells
In vitro, HK1.4 antibody can increase proliferation and stimulate cytokine release.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL volume, 10⁵–10⁸ cells/test).
Titrate antibody for optimal performance.
Fluorochrome Information:
Super Bright 436 is excited with the violet laser (405 nm) and emits at 436 nm.
Recommended filter: 450/50 bandpass or equivalent.
Ensure your instrument is compatible with this fluorochrome.
Buffer Recommendation:
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize nonspecific interactions.
Fixation:
Samples can be stored in:
- IC Fixation Buffer (Product # 00-8222)
- 1-step Fix/Lyse Solution (Product # 00-5333)
Storage: up to 3 days at 4°C in the dark with minimal signal loss.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Granulocytes are white blood cells containing cytoplasmic granules, also known as polymorphonuclear leukocytes (PMN/PML) due to their lobed nuclei.
They include neutrophils, the most abundant granulocytes, and are released from bone marrow under the regulation of complement proteins.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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