Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), eBioscience
Mouse CD127 (IL-7 receptor α) 인식하는 Rat IgG2a 단일클론 항체로, Flow Cytometry, IHC, IP, Functional Assay 등에 사용 가능. IL-7 결합 차단 기능 보유. 액상형, 0.5 mg/mL 농도, 4°C 보관.
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Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 2 publications |
| Immunocytochemistry (ICC/IF) | - | 3 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | 97 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | Assay-Dependent | - |
| Functional Assay | Assay-Dependent | 1 publication |
| In vitro Assay (IV) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A7R34 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467457 |
Available Conjugated Formats
- Alexa Fluor 488
- Alexa Fluor 561
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661 / 737 / 805
- Brilliant Violet 421 / 650 / 711
- eFluor 450 / 660
- FITC
- Functional Grade
- NovaFluor Red 700 / 710
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 645
- Custom conjugation available upon request
Product Specific Information
The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor.
IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes.
In the periphery, mature T cells express CD127 at low levels.
A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation.
Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.
Applications Reported: Flow cytometric analysis, immunoprecipitation, immunohistology staining of frozen tissue sections, and IL-7 blocking in functional studies.
(Use Functional Grade purified A7R34, Product #16-1271-85, in functional assays.)
Applications Tested: Tested by flow cytometric analysis of mouse thymocytes and splenocytes.
Recommended ≤0.5 µg per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD127 (Interleukin-7 receptor, IL-7R) is a glycoprotein involved in lymphopoiesis regulation.
The active receptor is an alpha/gamma chain heterodimer.
The gamma(c) chain activates signal transduction, while the alpha chain determines specific signaling events.
CD127 promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells.
Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive SCID.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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