Thermo Fisher Scientific Mouse anti-Human IgG Fc (CH2 domain) Secondary Antibody

Applications

Tested Dilution

Western Blot (WB)

0.5 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Flow Cytometry (Flow)

1/50 - 1/100

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Secondary Antibody

Clone

8A4

Immunogen

Human IgG polyclonal.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_931587

Target

IgG Fc

Antibody Form

Whole Antibody

Product Specific Information

This antibody recognizes an epitope within the CH2 domain of the Fc region of all subclasses of human and Cynomolgus monkey IgG. For Flow cytometry, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.