
Thermo Fisher Scientific Mouse anti-Human IgG Fc (CH2 domain) Secondary Antibody
인간 IgG Fc(CH2 도메인)를 인식하는 마우스 모노클로날 2차 항체. Western blot, IHC, Flow cytometry, ELISA 등 다양한 응용에 적합. 비결합(Unconjugated) 형태로 고순도 Protein A 정제. 인간 및 시노몰구스 원숭이 IgG 서브클래스에 반응.
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Thermo Fisher Scientific Mouse anti-Human IgG Fc (CH2 domain) Secondary Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Flow Cytometry (Flow) | 1/50 - 1/100 |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | 8A4 |
| Immunogen | Human IgG polyclonal |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_931587 |
| Target | IgG Fc |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody recognizes an epitope within the CH2 domain of the Fc region of all subclasses of human and Cynomolgus monkey IgG.
For flow cytometry, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting, or purification of their specified targets.
Secondary antibodies offer increased versatility, enabling users to employ various detection systems (e.g., HRP, AP, fluorescence). They also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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