Thermo Fisher Scientific TRMT12 Monoclonal Antibody (OTI1C9)
TRMT12 단백질을 인식하는 Mouse monoclonal antibody로 Western blot 및 IHC(P)에서 검증됨. Human 시료에 반응하며, 1 mg/mL 농도의 액상 형태로 제공. PBS 기반 버퍼에 BSA, glycerol 포함. -20°C 보관, 반복 동결·해동 피해야 함.
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Applications
- Western Blot (WB): 1:2,000
- Immunohistochemistry (Paraffin) (IHC (P)): 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C9 |
| Immunogen | Full length human recombinant protein of TRMT12 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2725577 |
Target Information
TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3′ position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome.
TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.
TYW2 was found to be amplified in 7 of 8 breast cancer cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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