Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioSN4 (SN4 C3-3A2)), PerCP-eFluor 710, eBioscience
인간 CD9 단백질을 인식하는 마우스 단클론 항체로 PerCP-eFluor 710 형광 표지. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 5 µL/test 사용. 4°C 암소 보관, 동결 금지. 다양한 세포 표면 CD9 발현 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioSN4 (SN4 C3-3A2) |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation/Emission Max | 482/708 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2762455 |
Additional Formats
Product Specific Information
Description:
The eBioSN4 monoclonal antibody reacts with human CD9, a 24 kDa member of the tetraspanin family characterized by four hydrophobic transmembrane domains. CD9 is expressed in platelets, eosinophils, basophils, pre-B cells, activated T cells, and neural cell lines. It is associated with malignant phenotypes, including expression in various leukemia types. CD9 plays roles in platelet aggregation, pre-B cell adhesion, and T-cell co-stimulation.
Applications Reported:
This antibody is reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human platelets. Use 5 µL (0.25 µg) per test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test.
Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with a 488 nm blue laser. Recommended filter: 710/50 bandpass (695/40 acceptable alternative). Ensure instrument compatibility.
Fixation:
Samples can be stored in:
- IC Fixation Buffer (Product #00-8222-49)
- 1-step Fix/Lyse Solution (Product #00-5333-54)
Store up to 3 days at 4°C in the dark with minimal impact on brightness or FRET efficiency.
Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Target Information
CD9 antigen is a glycoprotein expressed on B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated T lymphocytes, neurons, and glial cells. It belongs to the tetraspanin family with four transmembrane domains. CD9 regulates cell activation and aggregation via interaction with integrins (CD41/CD61). It is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, sperm-egg fusion, and T cell stimulation. CD9 expression is linked to suppression of cancer cell motility and metastasis and functions as a metastasis suppressor in solid tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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