Thermo Fisher Scientific HMGB1 Recombinant Rabbit Monoclonal Antibody (HL2059)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA5-50368 has successfully been used in Indirect ELISA.

Target Information

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA-binding chromosomal protein. Recent studies indicate that damaged or necrotic cells release HMGB1 into the extracellular space, where it functions as a proinflammatory cytokine.

Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains:

• Two positively charged DNA-binding domains: HMG box A (aa 9–79) and box B (aa 89–162)
• A negatively charged 30 aa C-terminal acidic tail region

Residues 28–44 and 180–185 contain a nuclear localization signal (NLS).
The cytokine activity resides in the B box, while the A box interacts with transcription factor helix-loop-helix domains.

HMGB1 functions as:

• An inflammatory mediator promoting monocyte migration and cytokine secretion
• A mediator of T cell–dendritic cell interactions
• A signal transducer via receptors RAGE, TLR2, and TLR4

It is highly conserved and ubiquitously expressed in nuclei and cytoplasm of most cell types, acting as a key mediator of inflammation during sterile and infection-associated responses.
In animal models, HMGB1 has been shown to trigger sterile inflammation via TLR4 and RAGE activation.
Elevated HMGB1 levels are associated with sterile tissue injury, infection, sepsis, arthritis, and ischemia-reperfusion injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.