Thermo Fisher Scientific GAPDH Polyclonal Antibody
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카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
PA585074 | - | Thermo Fisher Scientific PA585074 GAPDH Polyclonal Antibody 100 ul pk | 재고문의 | pk | 524,000원 | - | 576,400원 |
다른 상품 둘러보기
Applications
Tested Dilution
Publications
Western Blot (WB)
1:5,000-1:100,000
View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:1,000
Immunocytochemistry (ICC/IF)
1:100-1:1,000
Immunoprecipitation (IP)
1:100-1:500
Product Specifications
Species Reactivity
Bovine, Dog, Fruit fly, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Zebrafish
Published species
Human
Host/Isotype
Rabbit / IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human GAPDH.
Conjugate
Unconjugated Unconjugated Unconjugated
Form
Liquid
Concentration
0.08 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792223
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (94%), Rat (94%), Zebrafish (86%), Xenopus laevis (85%), Dog (95%), Cat (96%), Pig (95%), Rabbit (94%), Chicken (92%), Sheep (94%), Rhesus Monkey (99%), Bovine (94%), Guinea pig (94%).
Positive Control: 293T, A431, HeLa, HepG2, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, E.coli M15, SH-SY5Y, A549, U2OS, H9C2, A7R5, mouse skin tissue.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimers disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington
s disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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