Thermo Fisher Scientific Phospho-p38 MAPK (Thr180, Tyr182) Recombinant Rabbit Monoclonal Antibody (B10H8L5)

Applications

Product Specifications

Product Specific Information

Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

MAPK14 (p38 alpha) is a stress-activated protein kinase within the MAPK family. The p38 MAPK cascade regulates cellular responses to stress, inflammation, and other stimuli. p38 MAPK is inactive in its nonphosphorylated form and becomes rapidly activated by dual phosphorylation of the Thr-Gly-Tyr motif.

There are four isoforms of p38 MAPK, differing in tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to TNF, IL-1, heat shock, or other stimuli, activation of MAPK kinase-3 occurs via phosphorylation. Activated MAPK kinase-3/6 phosphorylate Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK subsequently activates transcription factors such as ATF-2, CHOP-1, and MEF-2 through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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