Thermo Fisher Scientific Phospho-Tau (Ser199) Recombinant Rabbit Monoclonal Antibody (2H23L4)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody is predicted to react with human based on sequence homology.
Intact IgG appears on a non-reducing gel as an approximately 150 kDa band and upon reduction generates a ~25 kDa light chain and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select optimal candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to the rabbit immune repertoire

Target Information

Tau is a neuronal microtubule-associated protein primarily localized on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules, while the N-terminus interacts with neural plasma membrane components, functioning as a linker between both.

In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building blocks of neurofibrillary lesions in Alzheimer’s disease (AD) brains. Hyper-phosphorylation impairs Tau’s microtubule-binding function, leading to destabilization and neuronal degeneration.

Tau phosphorylation is mediated by multiple kinases, including:

• GSK-3β
• Protein kinase A (PKA)
• Cyclin-dependent kinase 5 (cdk5)
• Casein kinase II

Hyper-phosphorylated Tau is implicated in several neurodegenerative disorders such as Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.