Thermo Fisher Scientific Phospho-IkB beta (Thr19, Ser23) Polyclonal Antibody
Human IkB beta 단백질의 Thr19 및 Ser23 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot에 적합하며, Protein A로 정제된 액상 형태. -20°C 보관, 방부제 무첨가. 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-IkB beta (Thr19, Ser23) Polyclonal Antibody
Applications
- Western Blot (WB)
Tested Dilution: 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues of human IkB beta surrounding pThr19/Ser23 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 8 µg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10986985 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins. Most agents that activate NF-kB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB.
The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex composed of three subunits: IKK alpha, IKK beta (catalytic subunits), and IKK gamma (regulatory subunit).
Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in IKK beta (Ser176 and Ser180 in IKK alpha), leading to conformational changes and kinase activation.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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