Thermo Fisher Scientific SPHK1 Monoclonal Antibody (OTI1A6), TrueMAB
SPHK1 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. Human 시료에 반응하며, lyophilized 형태로 제공. PBS buffer에 8% trehalose 포함, -20°C 보관 권장. 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A6 |
| Immunogen | Full length human recombinant protein of human SPHK1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine to produce sphingosine-1-phosphate (S1P), a key signaling molecule.
S1P interacts with G-protein coupled receptors and regulates various cellular processes including differentiation, calcium mobilization, and apoptosis.
SPHK inhibitors are being studied as potential cancer therapeutics.
S1P receptors belong to the EDG family of G protein-coupled receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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