Thermo Fisher Scientific KRT14 Monoclonal Antibody (OTI3C7), TrueMAB
인간 KRT14 단백질(aa 148-472)에 특이적인 마우스 단클론 항체. Western blot과 IHC(P)에서 검증됨. 동결건조 형태로 제공되며 PBS 버퍼(8% 트레할로스) 포함. 상피세포 구분 및 암 조직 연구용에 적합.
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Applications
- Western Blot (WB): 1:500
- Immunohistochemistry (Paraffin) (IHC (P)): 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3C7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 148–472 of human KRT14 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform desalting again (recommended: Zeba Spin Desalting Columns, 7 K MWCO, 0.5 mL, Product #89882).
Target Information
Cytokeratins (CK) are intermediate filaments found in epithelial cells, both keratinizing (e.g., skin) and non-keratinizing (e.g., mesothelial cells).
There are at least 20 CK types classified as type I (acidic) and type II (basic).
Keratin 14 (KRT14) belongs to the type I subfamily and pairs with keratin 5 to distinguish stratified from simple epithelial cells.
It is useful for identifying squamous cell carcinomas and is considered a prognostic marker in breast carcinoma.
Both acidic and basic keratins are expressed in all epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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